Facility Overview

Advanced Light Microscopy Core (ALMC)

The mission of the Advanced Light Microscopy Core is to facilitate labs from School of Medicine at University of Colorado Denver with access to advanced imaging research instrumentation at a reasonable price.

The core is currently housed within two buildings on the Anschutz Medical Campus: on 7th floor in Research 1 North and on 7th floor in Research 2. Training and support is provided for all ALMC instruments at no charge by a staff of three, consisting of a core administrator and two technicians.

The ALMC can assist you with fluorescent and label-free imaging of various cellular structures or tissues in several ways including:

Confocal Imaging – good for obtaining crisp images with good z-resolution and for 3D imaging.
TPE/SHG Imaging – use 2-photon excitation to visualize collagen/elastin and weak autofluorescent label-free structures to depths that can exceed 100μm.
TIRF Imaging – for imaging and monitoring events happening on the cell membranes (<100nm depth).
Real Time Confocal Imaging –for live cell imaging and monitoring fast dynamics within the cell, by using spinning disk confocal (up to 30fps).
CARS Imaging – for visualization of lipids, myelin and certain other cellular components label-free.
FCS/FCCS – Determine proteins diffusion properties, local concentration, size, binding and clustering.
RICS/N&B – Determine proteins diffusion properties, clustering and binding stoichiometry.
FRET – Determine when two molecules are in “close” proximity to one another for monitoring molecular interactions (FLIM-based FRET available).
Nomarski and fluorescence imaging combination – fluorescence labeling localization within cell structure.

Let us see if we can help you.

To set up an account, reserve an instrument or for assistance with any other item, please see the contact us page.

If you already have an account, you may proceed directly to the reservation page.

Advisory Committee

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