Please note the following facility policies:
User Policy The facility instruments are available only to trained users. Please contact us for scheduling training sessions. Each new user is entitled for two 2-hours training sessions (free of cost) for each instrument.
Billing Policy
- $25/hour for users working with PIs that are: NINDS-funded investigators, Neuroscience Program Members, or participants in recent Shared Instrumentation Grants that are supplying the core with new microscopes (3I-Spinning Disc, STED, and CARS)
- $35/hour for users working with PIs that are members of Colorado Clinical and Translational Sciences Institute. Join CCTSI
- $80/hour for all others
Cancellation Policy Users canceling reservations less than twenty-four hours in advance will be billed for at least one hour, up to the entire duration of the reservation if nobody else takes that time instead.
Equipment Policy Users may not add or modify computer hardware or software or microscope equipment. Please contact us if you have specific needs that can be accommodated.
By reserving microscope time you acknowledge and agree to the policies above. Violation may result in microscope access being revoked and/or billing for repair costs.
Please click on the microscope (and light source, if applicable) that you’d like to reserve.
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(room P15-7251, RC2)
- confocal and two-photon imaging
- multi-track and spectral acquisition
- FRET and ratiometric analysis, spectral decomposition
- non-descanned detectors for two-photon fluorescence and second harmonic generation
- lasers include: 30mW Argon (458nm, 477nm, 488nm, 514nm), 1mw 543nm, 5mW 633nm
- also uses Coherent Chameleon laser source
- Solent Scientific temperature incubation chamber
- uses Zen software
- see Related Resources for documentation
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(room P15-7251, RC2)
- confocal laser scanning imaging
- lasers: 405nm, 458nm, 488nm, 515nm, 543nm, 635nm
- CO2/humidity/temperature controlled chamber
- non-descanned detectors for two-photon, SHG and CARS
- capable of FCS, RICS and CARS
note: RESTRICTED USE for CARS, only with ALMC personnel assistance |
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Coherent Chameleon Ultra II
(room P15-7251, RC2)
- femtosecond pulsed light for two-photon excitation and second harmonic generation
- programmatically tunable from 680–1080nm, with up to 3.5W average power
- used with Zeiss LSM 510 META system
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(room P15-7244, RC2)
- total internal reflection fluorescence (TIRF) imaging
- video-rate, high sensitivity imaging with Quant EM:512SC camera
- lasers include: 200mW Argon (458nm, 477nm, 488nm, 514nm), 50mw 405nm, 150mW 561nm
- Zeiss Axiocam MRm camera also available
- PeCon XL-3-TIRF incubator
- uses AxioVision software
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(room P15-7244, RC2)
- corrected emission and excitation spectra
- fast and slow kinetics
- polarization and ratiometric measurements
- temperature control of sample
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(room P15-7243, RC2)
- basic bright field and fluorescence imaging
- 13 megapixel color camera
- uses AxioVision software
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(room P18-7402C, RC1)
- Olympus OBJECTIVES- U PLAN APO 10X oil N.A. 0.40, UAPO 40X oil N.A. 1.35, TIRFM PLAN APO 60X oil N.A. 1.45 WD 0.15mm, U PLAN APO 100X oil N.A. 1.35.
- EXCITATION SOURCE: 100 watt Hg lamp.
- CAMERA; Hamamatsu ORCA IIER monochromatic CCD camera 1344X1024 full chip, 6.45X6.45 um pixel .
- FILTER SETS: DAPI EX360-70 EM420-60, FITC EX450-80 EM535, TRITC EX535 EM635; CFP/YFP FRET filter set 8600 2v2(JP4)
- PLATFORM: Dell P4 3.2 GHz, Windows 2000 .
- DATA: ethernet, CD-W or DVD-W 16X.
- Acquisition SOFTWARE: Intelligent Imaging Slidebook v.4.067.
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(room P18-7402A1, RC1)
- OLYMPUS IX81 inverted motorized Microscope, Prior motorized XY stage, Environmental Chamber.
- Olympus OBJECTIVES: U PLAN Fluorite 10X N.A. 0.30 WD 10mm, LWD PLAN Fluorite 40X N.A. 0.60 WD 2.89-2.15 mm, TIRFM PLAN APO 60X oil N.A. 1.45 WD 0.15mm.
- EXCITATION SOURCE: Widefield fluorescence- 100 watt Hg lamp, TIRF laser illumination- Argon 10mW 488nm; 543 HeNe, 633 HeNe . CAMERA; Hamamatsu ORCA ER monochromatic CCD camera 1344X1024 full chip, 6.45X6.45 um pixel.
- FILTER SETS : Widefield Fluorescence- FITC EX480/40 EM 535/50, TRITC EX535/50 EM610/75 , Dual Band FITC/TRITC TIRF filters: 488 TIRFM Filter Set:Z-488\10 DMZ500RDC 515/50M , 543 TIRFM Filter Set Z-543\10 DMZ543RDC 590/50M, 633 TIRFM Filter Set Z-633\10 DMZ633RDC 700/75M
- PLATFORM: Dell P4 3.2 GHz, Windows 2000.
- DATA: ethernet, CD-W or DVD-W 16X.
- Acquisition SOFTWARE: Intelligent Imaging Slidebook v.4.067.
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(room P18-7206, RC1)
- Zeiss AXIOSKOP 2 FS MOT (Upright).
- Zeiss OBJECTIVES: 5X Fluar, N.A. 0.25, W.D. 12.2 mm, dry; 10X Plan-Neofluar, N.A. 0.3, W.D. 5.6 mm, dry; 25X Plan-Neofluar, N.A. 0.8, W.D. 0.21 mm, oil/water; 40X Achroplan, N.A. 0.8, W.D. 3.6 mm, water; 63X IR Achroplan, N.A. 0.9, W.D. 2.0 mm, water; 63X Apochromat, N.A. 1.4, W.D. 0.09 mm, oil.
- EXCITATION: Lasers: Argon 458nm, 477nm, 488nm, 514nm; HeNe 543nm; HeNe 633nm; Coherent Mira Ti:Sapphire tunable IR laser (720-900 nm) for 2-photon excitation. 2 Non-descan detectors; 3 Descan detectors; 1 Transmitted light detector (DIC capable).
- FILTER SETS: Wide range of filter options with 3 PMT detectors.
- PLATFORM: Siemens Pentium 4 1.7GHz processor (WINDOWS NT).
- DATA: CD-RW, Ethernet.
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Lasers on the Zeiss LSM 510
The Argon laser has four excitation lines (458, 477, 488, and 514 nm) and the two HeNe lasers offer additional lines at 543 nm and 643 nm. The IR 2 photon laser is a Coherent Mira 70 MHz mode locked Ti-Sapphire laser, tunable from ~720 nm to ~900 nm. |
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(room P18-7402A2, RC1)
- fast confocal imaging on Zeiss Axio Observer Z1 with Yokogawa CSU-X1
- lasers: 405, 445, 488, 515, 561 nm lines with millisecond 3I mSwitcher
- 3I mSAC millisecond spherical aberation correction
- 3I live specimen Vector Scanner
- Photometrics Evolve 16-bit EMCCD camera for confocal imaging
- Photometrics CoolSNAP HQ2 camera for wide-field imaging and FURA2 ratiometric Ca imaging
- Okolab cage incubator with temperature, CO2, air and humidity control
- uses 3I Slidebook 5.0 software
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(room P18-7402A2, RC1)
- fast confocal imaging on Zeiss Axio Examiner D1 with
- Yokogawa CSU-X1
- lasers: 405, 488, 561 nm lines with millisecond 3I mSwitcher
- 3I live specimen Vector Scanner
- Photometrics Evolve 16-bit EMCCD camera
- uses 3I Slidebook 5.0 software
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Microscopes may be reserved for any available time slot, but training and technical assistance may be available only at certain times.
