Facility Overview

Please note the following facility policies:

User Policy The facility instruments are available only to trained users. Please contact us for scheduling training sessions. Each new user is entitled for two 2-hours training sessions (free of cost) for each instrument.

Billing Policy

$15/hour for users working with PIs that are: NINDS-funded investigators
$25/hour for users working with PIs that are: Neuroscience Program Members, or participants in recent Shared Instrumentation Grants that are supplying the core with new microscopes (3I-Spinning Disc, STED, CARS, spectral/FLM lsm780)
$39/hour for users working with PIs that are members of Colorado Clinical and Translational Sciences Institute. Join CCTSI
$80/hour for all others
Also notice the overnight 9hr time slots and weekend time 7.5hrs (AM or PM) slots are available for long lapse experiments. We don't charge by hour for these long time slots, we charge by the booked time slot. The rates are:

$10/hour for users working with PIs that are: NINDS-funded investigators
$15/hour for users working with PIs that are: Neuroscience Program Members, or participants in recent Shared Instrumentation Grants that are supplying the core with new microscopes (3I-Spinning Disc, STED, CARS, spectral/FLM lsm780)
$20/hour for users working with PIs that are members of Colorado Clinical and Translational Sciences Institute. Join CCTSI

Cancellation Policy Users canceling reservations less than twenty-four hours in advance will be billed for at least one hour, up to the entire duration of the reservation if nobody else takes that time instead.

Equipment Policy Users may not add or modify computer hardware or software or microscope equipment. Please contact us if you have specific needs that can be accommodated.

By reserving microscope time you acknowledge and agree to the policies above. Violation may result in microscope access being revoked and/or billing for repair costs.

Done.

Advanced Light Microscopy Core (ALMC)

The mission of the Advanced Light Microscopy Core is to facilitate labs from School of Medicine at University of Colorado Denver with access to advanced imaging research instrumentation at a reasonable price.

The core is currently housed within two buildings on the Anschutz Medical Campus: on 7th floor in Research 1 North and on 7th floor in Research 2. Training and support is provided for all ALMC instruments at no charge.

The ALMC can assist you with fluorescent and label-free imaging of various cellular structures or tissues in several ways including, but not limited to:

Confocal Imaging – for obtaining crisp images with good z-resolution and for 3D imaging (diffraction limited resolution: 200nm in-plane and 500nm axial).
TPE/SHG (two-photon excitation / second harmonic generation) Imaging – for visualization of collagen/elastin and weak auto-fluorescent label-free structures to depths that can exceed hundreds of microns.
TIRF (total internal reflection fluorescence) Imaging – for imaging and monitoring events happening on the cell membranes (<100nm depth).
Real Time Confocal Imaging – for live cell imaging and monitoring fast dynamics within the cell, by using spinning disk confocal (up to 30fps).
CARS (coherent anti-stokes raman spectroscopy) Imaging – for visualization of lipids, myelin and certain other label-free cellular components.
FCS/FCCS (fluorescent (cross-)correlation spectroscopy) – for determining proteins diffusion properties, local concentration, size, binding and clustering.
RICS/N&B (raster imaging correlation spectroscopy / number & brightness analysis) – for determining proteins diffusion properties, clustering and binding stoichiometry.
Spectral detection imaging with un-mixing up to 8 different stains or fluorescent proteins spectra.
FLIM (fluorescent lifetime imaging) – for imaging based on fluorophore photon emission rather than standard wavelength (“color”) imaging; for NADH/FAD and other intrinsic auto-fluorescence entities in cells or tissue
FRET (förster resonance energy transfer) Imaging – for monitoring molecular interactions and proteins binding (FLIM-based FRET available).
STED (stimulation emission depletion) super-resolution imaging with resolution beyond diffraction limit, down to 50nm in-plane resolution, two-channel for co-localization.
3D STORM/PALM (super-resolution localization) that can achieve 20nm in-plane resolution and 50nm axial resolution.
Soon to have nSPIRO (modulation tracking) for super-resolution imaging of small moving objects (i.e. microvili, cilia, etc).

Let us see if we can help you.

To set up an account, learn how to reserve an instrument or for assistance with imaging needs for your project, please contact Radu Moldovan (303-724-3649, radu.moldovan@ucdenver.edu).

If you already have an account, you may proceed directly to the reservation page.

Advisory Committee

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